Southern blotting hybridization

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• Southern blotting technique — The procedure used to isolate and identify DNA fragments from a complex mixture. The isolated, denatured fragments are transferred from an agarose electrophoretic gel to a nitrocellulose filter and identified by hybridization with probes …   Dictionary of microbiology

• Southern blot — A Southern blot is a method routinely used in molecular biology to check for the presence of a DNA sequence in a DNA sample. Southern blotting combines agarose gel electrophoresis for size separation of DNA with methods to transfer the size… …   Wikipedia

• Hybridization probe — In molecular biology, a hybridization probe is a fragment of DNA or RNA of variable length (usually 100 1000 bases long), which is used in DNA or RNA samples to detect the presence of nucleotide sequences (the DNA target) that are complementary… …   Wikipedia

• Nucleic acid hybridization — Hybridization is the process, discovered by Alexander Rich, of combining complementary, single stranded nucleic acids into a single molecule. Nucleotides will bind to their complement under normal conditions, so two perfectly complementary… …   Wikipedia

• Southern Blot — Autoradiogramm eines Southern Blots Beim Southern Blot handelt es sich um eine 1975 von Edwin Southern entwickelte molekularbiologische Untersuchungsmethode für die DNA.[1] Sie ermöglicht den Nachweis einer Gensequenz in einem komplexen DNA… …   Deutsch Wikipedia

• blotting — General term for the transfer of protein, RNA or DNA molecules from a relatively thick acrylamide or agarose gel to a paper like membrane (usually nylon or nitrocellulose) by capillarity or an electric field, preserving the spatial arrangment.… …   Dictionary of molecular biology

• filter hybridization — any of various methods, e.g., Southern blotting, dot blotting, for detection of a specific nucleic acid sequence by immobilizing a population of single stranded nucleic acids on a filter, exposing it to a specific labeled single stranded nucleic… …   Medical dictionary

• Molecular biology — (pronounced /məˈlɛkjʊlər .../) is the branch of biology that deals with the molecular basis of biological activity. This field overlaps with other areas of biology and chemistry, particularly genetics and biochemistry. Molecular biology… …   Wikipedia

• Medical genetics — Clinical genetics redirects here. For the journal, see Clinical Genetics (journal). For a non technical introduction to the topic, see Introduction to Genetics. Part of a series on Genetics Key components Chromosome …   Wikipedia

• DNA microarray — A DNA microarray (also commonly known as gene chip, DNA chip, or biochip) is a collection of microscopic DNA spots attached to a solid surface. Scientists use DNA microarrays to measure the expression levels of large numbers of genes… …   Wikipedia

• genetic disease, human — Introduction       any of the diseases and disorders that are caused by mutations in one or more genes (gene).       With the increasing ability to control infectious and nutritional diseases in developed countries, there has come the realization …   Universalium